BRAVE TECHNOLOGY:
OPTO-FLUIDIC FORCE INDUCTION (OF2i)

Photons carry momentum, spin and angular momentum. And while they are only able to knock out our COO-boxer in our website’s introduction video, they are active and powerful forces in the nanoworld. At BRAVE Analytics we use these intrinsic light-properties – light forces – for our patented OptoFluidic Force Induction OF2i[1] technology to result in active, fast, and continuous online particle characterization. OF2i is arranged as a high throughput counting method for real-time process monitoring. The principle is based on the combination of very defined fluidic as well as optically-induced forces acting on particle streams[2].

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The minimal photonic forces are sufficient to deflect and (de)accelerate small particles from their fluidic induced motion, independent from their overlying Brownian motion. As a result, the induced particle trajectories contain size and shape information. The patented OF2i optical parallelization technology enables and actively sets thousands of particles in motion simultaneously for highly statistically valid readouts.

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Single particle visualization (slow motion)

Using an ultramicroscope setup, the actively altered particle trajectories can be determined and translated into characterization data with respect to sizes, size distributions and particle numbers (concentration) with a clear, statistically significant relation to the overall particle population. Due to the underlying active principles of OF2i, our measurements are orders of magnitude faster than Brownian motion processes used in DLS or NTA.

The OF2i technology operates as online and calibration-free process analytical technology (PAT). It is well established at the Medical University of Graz particle lab and tested in cooperation with partners from several industries and research labs.

 

[1] Hill, C. Optofluidic force induction (OF2i) - Platform technology for particle characterization and active manipulation in microfluidic environments (Doctoral dissertation). 2018, Medical University Graz.

[2] Kaneta, T., et al., Theory of optical chromatography. Analytical Chemistry, 1997. 69(14): p. 2701-2710.

A BRAVE VISION

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BRAVE  – Beyond the state of the art ...

The possibilities in the nano-world are endless and we are still discovering them. Our OF2i technology has created the revolutionary possibility to measure particles and samples on-line and continuously in the nanometer range with the help of u-fluidics and photonics. This method has been translated into a nanoparticle measuring instrument over the past two years.

Ing. Dr. Christian Hill, CTO and coordinator of OF2i-Technology, BRAVE Analytics GmbH

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BRAVE B1  – for online measurements

The prototype was tested, optimized and verified in various application areas. We are now taking the next step with various partners and the technology is being used on-line in production systems. The vision of "sample in – data out" on-line and in real-time, is within reach. 

Planned specifications of the measuring sensor – our vision

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* dependent on sample
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BRAVE B2  – for measurements in the lab

The BRAVE B2 nanoparticle analyzer was presented at Analytica 2022 in Munich and will be launched at the start of 2023. Test instruments are being used in medical research, to detect nanoplastics in water, monitor the production of surface coatings based on ceramic nanoparticles and in the manufacturing of hydroxyapatite solutions.

 

THE BRAVE DIFFERENCE

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SOURCE: ISO copyright office, ISO/TR 18196: Technical Report 2016; Nanotechnologies - Measurement technique matrix for the characterization of nano-objects. First Edition: 2016-11-15, Geneva p. 3-5

 

BRAVE CASES

SERVICES:

Studying biomolecular condensates and their role in health and diseases.

AIMS:

Time resolved liquid-liquid phase separation (LLPS) processes by observing the formation, concentration and size distribution of the proteins as they form, grow, and change over time.

Univ. Prof. Dr. Tobias Madl, Integrative Structural Biology and Metabolomics: "As the small condensates we are interested in cannot be detected with microscopy their study remains a blind spot. The OF2i technology promises to close this gap and to provide unprecedented insights into the processes driving early condensate formation and targeting of condensates.

AGENCY:

B2 benchtop instrument: Low Volume (<100µl) Time-Resolved Characterization

MEDICAL UNIVERSITY OF GRAZ

BIOPHYSICAL INSIGHTS

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SERVICES:

Device construction, installation, system verification

 

AIMS:

Process control for the wet-chemical production of nanocrystals (nano-hydroxy apatite) for re-mineralization of tooth enamel or treatment of bone defects

AGENCY:

On-line use of OF2i for process control compared to off-line measurements via TEM, NTA and DLS

FLUIDINOVA SA

CHEMICAL INDUSTRY

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SERVICES:

System integration, device design, process control

AIMS:

Aggregation control and process control of nano-ceramic surface coating systems for metals for low-friction, antibacterial, self-cleaning and chemically inert coatings.

AGENCY:

Use of OF2i for real-time measurement of coating processes compared to DLS and TEM

CREATIVE NANO PC

COATINGS

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SERVICES:

Sample particle characterization, system integration

 

AIMS:

Process control for the wet-chemical production of nanolignin in the end products "water-based paint" and "sun cream" and verification of industrial applicability.

 

AGENCY:

On-line use of OF2i for upstream and downstream process control compared to off-line measurements via NTA and DLS

LIGNOVATIONS

BIO BASED NANOLIGNIN PRODUCTION

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BRAVE PUBLICATIONS